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KMID : 1007520190280010165
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Food Science and Biotechnology
2019 Volume.28 No. 1 p.165 ~ p.174
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Enzymatic modification of daidzin using heterologously expressed amylosucrase in Bacillus subtilis
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Kim Eun-Ryoung
Rha Chan-Su
Jung Young-Sung
Choi Jung-Min
Kim Gi-Tae
Jung Dong-Hyun
Kim Tae-Jip
Seo Dong-Ho
Kim Dae-Ok
Park Cheon-Seok
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Abstract
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Amylosucrases (ASase, EC 2.4.1.4) from Deinococcus geothermalis (DGAS) and Neisseria polysaccharea (NPAS) were heterologously expressed in Bacillus subtilis. While DGAS was successfully expressed, NPAS was not. Instead, NPAS was expressed in Escherichia coli. Recombinant DGAS and NPAS were purified using nickel-charged affinity chromatography and employed to modify daidzin to enhance its water solubility and bioavailability. Analyses by LC/MS revealed that the major products of transglycosylation using DGAS were daidzein diglucoside and daidzein triglucoside, whereas that obtained by NPAS was only daidzein diglucoside. The optimal bioconversion conditions for daidzein triglucoside, which was predicted to have the highest water-solubility among the daidzin derivatives, was determined to be 4% (w/v) sucrose and 250 mg/L daidzin in sodium phosphate pH 7.0, with a reaction time of 12 h. Taken together, we suggest that the yield and product specificity of isoflavone daidzin transglycosylation may be modulated by the source of ASase and reaction conditions.
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KEYWORD
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Amylosucrase, Deinococcus geothermalis, Daidzin, Transglycosylation, Bacillus subtilis
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